{"id":2754,"date":"2019-03-24T12:36:00","date_gmt":"2019-03-24T17:36:00","guid":{"rendered":"https:\/\/irrf.org\/?p=2754"},"modified":"2022-07-19T10:04:06","modified_gmt":"2022-07-19T15:04:06","slug":"cell-press-structure-of-the-decorated-ciliary-doublet-microtubule","status":"publish","type":"post","link":"https:\/\/irrf.org\/cell-press-structure-of-the-decorated-ciliary-doublet-microtubule\/","title":{"rendered":"CELL PRESS: Structure of the Decorated Ciliary Doublet Microtubule"},"content":{"rendered":"\r\n\r\n\r\n

\"\"<\/a>AUTHORS<\/strong>:\u00a0 Meisheng Ma,\u00b9 Mihaela Stoyanova,\u00b2 Griffin Rademacher,\u00b3 Susan K. Dutcher,\u00b2 Alan Brown<\/strong>,\u00b3 and Rui Zhang,\u00b9. CELL PRESS, Article | Volume 179, Issue 4, P909-922.E12, October 31, 2019.<\/p>\r\n\r\n\r\n\r\n

\u00b9Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, School of Medicine, St. Louis, MO, USA<\/p>\r\n\r\n\r\n\r\n

\u00b2Department of Genetics, Washington University in St. Louis, MO, USA<\/p>\r\n\r\n\r\n\r\n

\u00b3Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA<\/p>\r\n\r\n\r\n\r\n

IN BRIEF: <\/strong>Visualizing axonemal microtubules and the proteins that decorate them, on the outside and inside, points to how the underlying periodic architecture supports cilia function.<\/p>\r\n\r\n\r\n\r\n

SUMMARY: <\/strong>The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells.\u00a0 In humans, impaired axoneme function causes a range of ciliopathies.\u00a0 Axoneme assembly, structure, and motility require a radially arranged set of doublet microtubules, each decorated in repeating patterns with non-tubulin components.\u00a0 Single-particle cryo-electron microscopy is used to visualize and build an atomic model of the repeating structure of a native axonemal doublet microtubule, which reveals the identities, positions, repeat lengths, and interactions of 38 associated proteins, including 33 microtubule inner proteins (MIPs).\u00a0 The structure demonstrates how these proteins establish the unique architecture of doublet microtubules, maintain coherent periodicities along the axoneme, and stabilize the microtubules against the repeated mechanical stress induced by ciliary motility.\u00a0 This work elucidates the architectural principles that underpin the assembly of this large, repetitive eukaryotic structure and provides a molecular basis for understanding the etiology of human ciliopathies.<\/p>\r\n\r\n\r\n\r\n

\r\n
\r\n
\"Headshot
Alan Brown, PhD<\/figcaption><\/figure>\r\n<\/figure>\r\n<\/div>\r\n\r\n\r\n\r\n

This study was conducted with IRRF<\/a> support \u2013 Alan Brown, PhD, 2018 and 2019.<\/strong><\/p>\r\n\r\n\r\n\r\n

Dr. Brown is a member of the faculty of the Department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School in Boston.\u00a0 He holds a B.Sc. in Biochemistry from the University of Warwick, Coventry, UK; and a PhD in Biochemistry from the University of Cambridge, Cambridge, UK<\/p>\r\n","protected":false},"excerpt":{"rendered":"

Visualizing axonemal microtubules and the proteins that decorate them, on the outside and inside, points to how the underlying periodic architecture supports cilia function.<\/p>\n","protected":false},"author":4,"featured_media":3612,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[340,219,220],"tags":[157,158],"_links":{"self":[{"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/posts\/2754"}],"collection":[{"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/comments?post=2754"}],"version-history":[{"count":1,"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/posts\/2754\/revisions"}],"predecessor-version":[{"id":4986,"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/posts\/2754\/revisions\/4986"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/media\/3612"}],"wp:attachment":[{"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/media?parent=2754"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/categories?post=2754"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/irrf.org\/wp-json\/wp\/v2\/tags?post=2754"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}